3I2D

Crystal Structure of S. Cerevisiae SUMO E3 Ligase SIZ1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	44.487, 88.980, 105.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.020 - 2.600
Rwork	0.211
R-free	0.25900
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.020	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.073	0.434
Number of reflections	13595
<I/σ(I)>	22	2.6
Completeness [%]	97.8	89.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	3.5 M NaCl, 100 mM HEPES 7.5 and either 5% glycerol or xylitol, VAPOR DIFFUSION, HANGING DROP, temperature 288K