3I2C
Crystal structure of anti-IL-23 antibody CNTO4088
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-03 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 4 |
| Unit cell lengths | 99.430, 99.430, 115.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.19783 |
| Rwork | 0.196 |
| R-free | 0.23503 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eo8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.310 |
| Data reduction software | d*TREK (9.6L) |
| Data scaling software | d*TREK (9.6L) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.169 | 0.549 |
| Number of reflections | 13889 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293 | 0.1 M CITRATE PH 3.5, 24% PEG 8K, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
