3I1G

Crystal structure of a GCN4 leucine zipper mutant at 1.6 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	95
Detector technology	CCD
Collection date	2005-03-31
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1000
Spacegroup name	P 32 2 1
Unit cell lengths	31.145, 31.145, 57.324
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.640 - 1.600
R-factor	0.257
Rwork	0.257
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	A STANDARD HELIX OF 28 POLYALANINE RESIDUES
RMSD bond length	0.005
RMSD bond angle	0.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.640	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	7098
<I/σ(I)>	20.7	2
Completeness [%]	85.9	23.6
Redundancy	3.2	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	Protein solution: 8 mg/mL Leucine zipper peptide, 0.1 M Sodium acetate pH 4.5. Reservoir solution: 0.2 M CaCl2, 0.1 M Sodium acetate pH 4.5, 20 % v/v Isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K