3I19
1.4 Angstrom Crystal Structure of Fluorescent Protein Cypet
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0082 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.873, 63.020, 71.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.140 - 1.360 |
| R-factor | 0.16907 |
| Rwork | 0.168 |
| R-free | 0.19693 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qyo |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.164 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.380 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.047 | 0.194 |
| Total number of observations | 550430 | |
| Number of reflections | 49295 | |
| <I/σ(I)> | 41 | 6.1 |
| Completeness [%] | 99.0 | 91.6 |
| Redundancy | 5.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 4000, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
