3I0X
Crystal structure of Clostridium acetobutylicum 8-oxoguanine glycosylase/lyase in complex with dsDNA containing adenine opposite to 8-oxoG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-15 |
| Detector | ADSC Q315R |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 92.130, 92.130, 190.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.950 - 1.800 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.085 | 0.490 |
| Number of reflections | 41903 | 3368 |
| <I/σ(I)> | 19.55 | 2.89 |
| Completeness [%] | 92.8 | 82.7 |
| Redundancy | 21.5 | 22.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 277 | PEG-4000 16%, 0.1M NaAcetate, 0.05M MgCl2, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
