3I0Q
Crystal Structure of the AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.466, 74.466, 135.526 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.720 - 2.800 |
| R-factor | 0.262 |
| Rwork | 0.262 |
| R-free | 0.34600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i1a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.720 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 10517 | |
| <I/σ(I)> | 34.2 | 12.3 |
| Completeness [%] | 93.6 | 100 |
| Redundancy | 10.6 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG3350, calcium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
