3I0P
Crystal structure of malate dehydrogenase from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Collection date | 2009-05-06 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 106.230, 106.230, 72.114 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.780 - 2.600 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v9n |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.208 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.780 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.144 | 0.536 |
| Number of reflections | 14659 | |
| <I/σ(I)> | 13.75 | 2.18 |
| Completeness [%] | 99.1 | 94.7 |
| Redundancy | 7.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | JCSG+ sparse matrix screen condition a2, 20% PEG 3000, 0.1 M Na Citrate pH 5.5, 25% glycerol as cryo-protectant, 30 mg/mL protein, crystal ID 203003a2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
