3I0M
Structure of the S. pombe Nbs1 FHA/BRCT-repeat domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9698 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 55.523, 62.385, 93.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.2258 |
| Rwork | 0.224 |
| R-free | 0.26780 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.198 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.097 | 0.450 |
| Number of reflections | 19822 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 8% PEG6000, 0.1M MES, 0.1M MgCl2, pH6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
