3HZP
Crystal structure of NTF2-like protein of unknown function MN2A_0505 from Prochlorococcus marinus (YP_291699.1) from Prochlorococcus sp. NATL2A at 1.40 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-18 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91162,0.97929,0.97918 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 58.046, 58.046, 63.620 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.025 - 1.400 |
R-factor | 0.168 |
Rwork | 0.167 |
R-free | 0.18400 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.527 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.025 | 29.020 | 1.440 |
High resolution limit [Å] | 1.400 | 6.260 | 1.400 |
Rmerge | 0.075 | 0.045 | 0.748 |
Total number of observations | 1473 | 9847 | |
Number of reflections | 24917 | ||
<I/σ(I)> | 6.295 | 14.2 | 1 |
Completeness [%] | 100.0 | 98.5 | 100 |
Redundancy | 5.4 | 4.6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 40.0000% PEG-600, 0.1M Citrate pH 5.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |