3HZO
Rv0554 from Mycobacterium tuberculosis - the structure solved from the tetragonal crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-02 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 100.779, 100.779, 135.112 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.166 |
| Rwork | 0.163 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.921 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.131 | 0.318 |
| Number of reflections | 21551 | |
| <I/σ(I)> | 20.444 | 3.1 |
| Completeness [%] | 97.8 | 79.8 |
| Redundancy | 10.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 95% (0.1 M NaAc pH 4.92, 5% MPD, 10% Ethylene glycol) and 5 % Tascimate pH8.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
