Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 4 2 2 |
| Unit cell lengths | 167.250, 167.250, 62.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 118.260 - 2.980 |
| R-factor | 0.265 |
| Rwork | 0.265 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dnv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.700 | 3.100 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.430 | |
| Number of reflections | 18730 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | tascimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | tascimate | ||
| 2 | 1 | 2 | tascimate |
