3HZH
Crystal structure of the CheX-CheY-BeF3-Mg+2 complex from Borrelia burgdorferi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2008-02-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97923 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 63.705, 63.705, 175.685 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.640 - 1.960 |
Rwork | 0.248 |
R-free | 0.25900 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 2.021 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | RESOLVE (ShelxD) |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.640 | 1.980 |
High resolution limit [Å] | 1.960 | 1.960 |
Number of reflections | 45929 | |
<I/σ(I)> | 26 | 3.1 |
Completeness [%] | 93.6 | 96 |
Redundancy | 6 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | Ammonium Sulfate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |