3HZB
Crystal structure of a betagamma-crystallin domain from Flavobacterium johnsoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-09-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81480 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 53.647, 99.279, 64.101 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.740 |
| Rwork | 0.192 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nps |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.170 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.800 |
| High resolution limit [Å] | 1.740 | 3.750 | 1.740 |
| Rmerge | 0.075 | 0.026 | 0.536 |
| Number of reflections | 69130 | ||
| <I/σ(I)> | 14.856 | 2.19 | |
| Completeness [%] | 99.8 | 98.5 | 100 |
| Redundancy | 3.8 | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 30% PEG 8K, 0.1M NaCacodylate,0.4M KCl, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
