3HZ4
Crystal Structure of Thioredoxin from Methanosarcina mazei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 82.068, 82.068, 93.874 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.19335 |
| Rwork | 0.191 |
| R-free | 0.24659 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.086 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.130 | 0.390 |
| Number of reflections | 18041 | |
| <I/σ(I)> | 9.9 | 9.6 |
| Completeness [%] | 94.8 | 100 |
| Redundancy | 21.5 | 21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 20% Peg 3350, 0.1M Bis Tris pH 5.5, 0.2M LiSo4, 0.1M EDTA, 20% Ethylene Glycol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
