3HYT
Structural Basis of GDP Release and Gating in G Protein Coupled Fe2+ Transport
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 293 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.000, 46.410, 120.070 |
Unit cell angles | 90.00, 94.95, 90.00 |
Refinement procedure
Resolution | 45.080 - 2.740 |
R-factor | 0.23045 |
Rwork | 0.227 |
R-free | 0.29398 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.820 |
High resolution limit [Å] | 2.720 | 2.720 |
Completeness [%] | 96.7 | 82 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% PEG, 100mM MgCl2, 1mM mant-GMPPNP, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |