3HYQ
Crystal Structure of Isopentenyl-Diphosphate delta-Isomerase from Salmonella entericase
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-31 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.443, 64.152, 35.187 |
| Unit cell angles | 90.00, 101.36, 90.00 |
Refinement procedure
| Resolution | 25.005 - 1.525 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.18700 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.536 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.4_58) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Number of reflections | 26450 | |
| <I/σ(I)> | 19 | 2.1 |
| Completeness [%] | 98.1 | 78 |
| Redundancy | 3.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.4 M ammonium dihydrogen phosphate, chymotripsyn (1:100), VAPOR DIFFUSION, SITTING DROP, temperature 289K |
