3HYL
Crystal Structure of Transketolase from Bacillus anthracis
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-04 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.801, 70.975, 145.795 |
| Unit cell angles | 90.00, 117.35, 90.00 |
Refinement procedure
| Resolution | 36.350 - 2.160 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.532 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.350 | 2.200 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.372 | |
| Number of reflections | 67767 | |
| <I/σ(I)> | 13.6 | 4.76 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 4.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 291 | 0.2 M Magnesium formate pH 5.9, 20 % PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
