3HYI
Crystal structure of full-length DUF199/WhiA from Thermatoga maritima
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-05-08 |
Detector | R-AXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.648, 61.303, 97.215 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.400 - 2.340 |
R-factor | 0.23014 |
Rwork | 0.228 |
R-free | 0.27608 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | N-terminal "LAGLIDADG" domain of T. maritima DUF199/WhiA |
RMSD bond length | 0.005 |
RMSD bond angle | 0.868 |
Data reduction software | HKL-2000 |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.420 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmerge | 0.053 | 0.347 |
Number of reflections | 13400 | |
<I/σ(I)> | 27 | 5 |
Completeness [%] | 98.6 | 98.3 |
Redundancy | 4.7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1 M Tris, pH 8.0, 0.2 M NaCl, 10% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |