3HYI
Crystal structure of full-length DUF199/WhiA from Thermatoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-08 |
| Detector | R-AXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.648, 61.303, 97.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.400 - 2.340 |
| R-factor | 0.23014 |
| Rwork | 0.228 |
| R-free | 0.27608 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | N-terminal "LAGLIDADG" domain of T. maritima DUF199/WhiA |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.868 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.053 | 0.347 |
| Number of reflections | 13400 | |
| <I/σ(I)> | 27 | 5 |
| Completeness [%] | 98.6 | 98.3 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1 M Tris, pH 8.0, 0.2 M NaCl, 10% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
