3HY2
Crystal Structure of Sulfiredoxin in Complex with Peroxiredoxin I and ATP:Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-11 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.330, 92.410, 131.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.380 - 2.100 |
R-factor | 0.22641 |
Rwork | 0.224 |
R-free | 0.27282 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.473 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 41701 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 293 | 7-9.5% PEG 6000, 100 mM HEPES pH 7.6, VAPOR DIFFUSION, temperature 293K |