3HX1
Crystal structure of the Slr1951 protein from Synechocystis sp. Northeast Structural Genomics Consortium Target SgR167A
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-14 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97931, 0.97907, 0.91162 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 56.130, 56.130, 290.868 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.300 - 2.500 |
Rwork | 0.232 |
R-free | 0.24400 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXDE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.141 | 0.629 |
Number of reflections | 17793 | |
<I/σ(I)> | 21.8 | 2.3 |
Completeness [%] | 99.4 | 99.6 |
Redundancy | 9.4 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under oil | 8 | 291 | 40 % PEG 1000, 0.1M potassium phosphate monobasic, 0.1M TrisHCl, pH 8.0, Microbatch under oil, temperature 291K |