3HWW
Crystal structure of menaquinone synthesis protein MenD from E. coli in complex with oxoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 117.910, 117.910, 175.276 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.320 - 1.950 |
| R-factor | 0.21104 |
| Rwork | 0.209 |
| R-free | 0.25453 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jlc |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.973 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.080 | 0.180 |
| Number of reflections | 80890 | |
| <I/σ(I)> | 18.2 | 1.85 |
| Completeness [%] | 94.2 | 84.9 |
| Redundancy | 8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.5-0.6M tri-Na citrate, 1mM oxoglutarate, 5mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
