3HWL
Crystal Structure of T4 lysozyme with the unnatural amino acid p-Acetyl-L-Phenylalanine incorporated at position 131
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-09-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.972, 59.972, 96.282 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.307 - 1.800 |
R-factor | 0.1885 |
Rwork | 0.187 |
R-free | 0.22680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l63 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.915 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.940 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 19132 | |
<I/σ(I)> | 30.4 | 10.6 |
Completeness [%] | 99.8 | 100 |
Redundancy | 14.4 | 15.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 2.0M sodium/potassium phosphate, 0.2M sodium chloride, 0.04% sodium azide, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |