3HWJ
Crystal structure of the second PHR domain of Mouse Myc-binding protein 2 (MYCBP-2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-13 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.877, 99.922, 83.054 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.940 - 2.250 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Poly ala based on the PDB Code 3GBW |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.715 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 18914 | |
| <I/σ(I)> | 8.7 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 2000mM DL Malic acid, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
