3HW6
Crystal structure of avian influenza virus PA_N in complex with Mn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Detector technology | CCD |
Collection date | 2009-03-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 51.504, 60.211, 67.215 |
Unit cell angles | 96.06, 96.49, 109.66 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.24283 |
Rwork | 0.240 |
R-free | 0.28870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ebj |
RMSD bond length | 0.019 |
RMSD bond angle | 1.818 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.080 | 0.303 |
Number of reflections | 19014 | |
Completeness [%] | 98.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 17.5% PEG3350(w/v), 100mM MgAc, 100mM MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |