3HW3
The crystal structure of avian influenza virus PA_N in complex with UMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-20 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 51.132, 59.695, 66.850 |
Unit cell angles | 96.28, 96.65, 109.44 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.22056 |
Rwork | 0.218 |
R-free | 0.27593 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ebj |
RMSD bond length | 0.016 |
RMSD bond angle | 1.639 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.251 | |
Number of reflections | 54981 | |
Completeness [%] | 99.0 | 95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 17.5% PEG3350(w/v), 100mM MgAc, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |