3HW2
Crystal structure of the SifA-SKIP(PH) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.939 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 91.799, 110.866, 44.274 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.300 |
| R-factor | 0.2473 |
| Rwork | 0.240 |
| R-free | 0.30925 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cxb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.338 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Total number of observations | 31607 | |
| Number of reflections | 7176 | |
| <I/σ(I)> | 8.9 | 3 |
| Completeness [%] | 99.3 | 95.8 |
| Redundancy | 4.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 30% PEG 4000, 0.2M Na acetate trihydrate, 0.1 M Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
