3HVN
Crystal structure of cytotoxin protein suilysin from Streptococcus suis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 170 |
Detector technology | CCD |
Collection date | 2008-10-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 95.720, 95.720, 117.046 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.409 - 2.852 |
R-factor | 0.28 |
Rwork | 0.279 |
R-free | 0.29400 |
Structure solution method | MAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.475 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
High resolution limit [Å] | 2.850 | 6.140 | 2.850 |
Rmerge | 0.052 | 0.043 | 0.316 |
Number of reflections | 13204 | ||
<I/σ(I)> | 30.364 | ||
Completeness [%] | 88.5 | 85.5 | 66.4 |
Redundancy | 5.7 | 5.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 289 | 8% polyethylene glycol (PEG)3350, 0.12M sodium citrate (pH7.0), 5% 1,2,3-heptanetriol isomer H, 2% 1,1,1,3,3,3-hexafluoro-2-propanol, temperature 289K | |
2 |