3HVM
Agmatine Deiminase from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-24 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 52.082, 52.082, 246.398 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.370 - 2.100 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cmu |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.853 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.370 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.043 | 0.143 |
| Number of reflections | 18081 | |
| <I/σ(I)> | 31.053 | 16.5 |
| Completeness [%] | 76.0 | 59.8 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 15% PEG 4K, 10 mM sodium citrate pH 5.6, and 7% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
