3HV9
Crystal structure of FimX EAL domain from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 107.900, 107.900, 131.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.722 - 2.298 |
| R-factor | 0.1973 |
| Rwork | 0.195 |
| R-free | 0.23660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3HVB |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.106 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.732 | 2.430 |
| High resolution limit [Å] | 2.298 | 2.298 |
| Rmerge | 0.062 | 0.494 |
| Number of reflections | 20719 | |
| <I/σ(I)> | 31.7 | 4.4 |
| Completeness [%] | 99.8 | 97.9 |
| Redundancy | 11.8 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | Ammonium sulfate, PEG 8000, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
