3HV8
Crystal structure of FimX EAL domain from Pseudomonas aeruginosa bound to c-di-GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9180 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.950, 93.950, 68.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.540 - 1.445 |
| R-factor | 0.1925 |
| Rwork | 0.192 |
| R-free | 0.21180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3HVB |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.171 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.100 | 1.560 |
| High resolution limit [Å] | 1.445 | 1.445 |
| Rmerge | 0.053 | 0.619 |
| Number of reflections | 54441 | |
| <I/σ(I)> | 25.6 | 3.5 |
| Completeness [%] | 98.4 | 92.3 |
| Redundancy | 12.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | Ammonium sulfate, PEG 8000, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
