3HV5
Human p38 MAP Kinase in Complex with RL24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-17 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.54170 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.770, 74.120, 71.600 |
| Unit cell angles | 90.00, 93.72, 90.00 |
Refinement procedure
| Resolution | 29.950 - 2.250 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zyj |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.613 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.350 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.046 | 0.250 |
| Number of reflections | 34895 | 4250 |
| <I/σ(I)> | 19.77 | 4.5 |
| Completeness [%] | 99.0 | 99.3 |
| Redundancy | 2.65 | 2.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES, 20-30% PEG4000, 50 mM n-BOG, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
