3HV5
Human p38 MAP Kinase in Complex with RL24
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-05-17 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.54170 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.770, 74.120, 71.600 |
Unit cell angles | 90.00, 93.72, 90.00 |
Refinement procedure
Resolution | 29.950 - 2.250 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zyj |
RMSD bond length | 0.016 |
RMSD bond angle | 1.613 |
Data scaling software | XSCALE |
Phasing software | PHASER (1.3.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.350 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.046 | 0.250 |
Number of reflections | 34895 | 4250 |
<I/σ(I)> | 19.77 | 4.5 |
Completeness [%] | 99.0 | 99.3 |
Redundancy | 2.65 | 2.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES, 20-30% PEG4000, 50 mM n-BOG, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |