3HV1
Crystal structure of a polar amino acid ABC uptake transporter substrate binding protein from Streptococcus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.559, 78.563, 80.030 |
| Unit cell angles | 90.00, 105.41, 90.00 |
Refinement procedure
| Resolution | 45.020 - 1.900 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.020 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.055 | 0.378 |
| Number of reflections | 44250 | |
| <I/σ(I)> | 19.5 | 2.5 |
| Completeness [%] | 98.7 | 97.9 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2M L-Proline, 0.1M HEPES, 10% Polyethylene glycol monomethyl ether 2000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
