3HV0
Tryptophanyl-tRNA synthetase from Cryptosporidium parvum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-15 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.320, 85.220, 73.110 |
| Unit cell angles | 90.00, 101.91, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.420 |
| R-factor | 0.22923 |
| Rwork | 0.227 |
| R-free | 0.27286 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PED ENTRY 3FOC |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.198 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.070 |
| High resolution limit [Å] | 2.420 |
| Rmerge | 0.096 |
| Number of reflections | 25491 |
| <I/σ(I)> | 9.7 |
| Completeness [%] | 95.5 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 0.25 M MgCl2, 0.1 M Bis/Tris pH 6.0, 28% PEG 3350, 10mM ATP, 1mM TCEP, 2mM L-tryptophan, vapor diffusion, temperature 293K |
