3HUU
Crystal structure of transcription regulator like protein from Staphylococcus haemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.872, 68.080, 97.835 |
| Unit cell angles | 82.42, 75.95, 79.39 |
Refinement procedure
| Resolution | 43.760 - 1.950 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.27400 |
| Structure solution method | SAD |
| Starting model (for MR) | None |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD (& SHARP) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.060 | 0.430 |
| Number of reflections | 82791 | |
| <I/σ(I)> | 10.5 | 3.5 |
| Completeness [%] | 89.7 | 96.8 |
| Redundancy | 7.5 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 10% PEG 8K, 8% Ethylene Glycol, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
