3HTQ
the hemagglutinin structure of an avian H1N1 influenza A virus in complex with LSTc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | I 2 3 |
Unit cell lengths | 198.443, 198.443, 198.443 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.610 - 2.955 |
R-factor | 0.2182 |
Rwork | 0.218 |
R-free | 0.26090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ru7 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.339 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS (1.21) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.060 |
High resolution limit [Å] | 2.955 | 2.950 |
Rmerge | 0.199 | 0.570 |
Number of reflections | 27329 | |
<I/σ(I)> | 21.3 | 8 |
Completeness [%] | 99.5 | 96.5 |
Redundancy | 21.7 | 17.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 295 | 100mM HEPES, 40% PEG 400, 120mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K, pH 6.8 |