3HT2
Zink containing polyketide cyclase RemF from Streptomyces resistomycificus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-14 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.282 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.433, 97.255, 47.743 |
| Unit cell angles | 90.00, 91.99, 90.00 |
Refinement procedure
| Resolution | 48.620 - 2.000 |
| R-factor | 0.19577 |
| Rwork | 0.193 |
| R-free | 0.24440 |
| Structure solution method | SAD |
| Starting model (for MR) | Native RemF |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.339 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.620 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.071 | 0.144 |
| Number of reflections | 20594 | |
| <I/σ(I)> | 23.4 | 8.6 |
| Completeness [%] | 97.0 | 82 |
| Redundancy | 6.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1M tri-sodium citrate, 0.1M sodium cacodylate, 0.2M ZnCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
