3HSQ
Structural Basis for the Sugar Nucleotide and Acyl Chain Selectivity of Leptospira interrogans LpxA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-07-15 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 109.149, 109.149, 117.124 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.360 - 2.100 |
R-factor | 0.21736 |
Rwork | 0.215 |
R-free | 0.26048 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lxa |
RMSD bond length | 0.009 |
RMSD bond angle | 1.170 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 26.360 |
High resolution limit [Å] | 2.100 |
Number of reflections | 44733 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 20% isopropanol, 1M sodium citrate, 20% polyethylene glycol 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |