3HSE
Crystal structure of Staphylococcus aureus protein SarZ in reduced form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-06 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 60.830, 60.830, 192.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.710 - 2.900 |
| Rwork | 0.286 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hrm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.299 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.710 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.048 | 0.496 |
| Number of reflections | 8051 | |
| <I/σ(I)> | 25.2 | |
| Redundancy | 5.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3 | 298 | 3.2 M ammonium sulfate, 0.1 M citric acid , pH 3.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
