3HS9
Intersectin 1-peptide-AP2 beta ear complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.088, 47.103, 58.945 |
| Unit cell angles | 90.00, 97.66, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.251 |
| Rwork | 0.249 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g30 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.564 |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.523 | 58.420 | 2.060 |
| High resolution limit [Å] | 1.950 | 6.170 | 1.950 |
| Rmerge | 0.045 | 0.031 | 0.466 |
| Total number of observations | 2262 | 12974 | |
| Number of reflections | 23441 | ||
| <I/σ(I)> | 9.119 | 19.9 | 1.6 |
| Completeness [%] | 96.4 | 81.4 | 96.3 |
| Redundancy | 3.8 | 3.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 18% PEG8000, 100mM HEPES, 4mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
