3HS8
Intersectin 1-peptide-AP2 alpha ear complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 64.484, 120.412, 32.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.100 - 1.900 |
R-factor | 0.191 |
Rwork | 0.189 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.645 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.030 | 0.019 | 0.130 |
Number of reflections | 19195 | ||
<I/σ(I)> | 27.513 | ||
Completeness [%] | 93.3 | 94.4 | 83.2 |
Redundancy | 2.9 | 2.9 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 20% PEG4000, 100mM HEPES, 10% Isopropanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |