3HS2
Crystal structure of PHD truncated to residue 57 in an orthorhombic space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 107.001, 122.542, 61.174 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.250 - 2.200 |
| R-factor | 0.2305 |
| Rwork | 0.227 |
| R-free | 0.27060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hry |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.777 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.250 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.110 | 0.321 |
| Number of reflections | 20810 | |
| <I/σ(I)> | 9 | 6.87 |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 18% PEG 8000, 0.2M LiSO4, 0.1M Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
