3HS1
Structure of Holliday junction formed by d(CCGGTACCGG); Crystal grown with CoCl2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.002, 24.760, 37.369 |
| Unit cell angles | 90.00, 110.62, 90.00 |
Refinement procedure
| Resolution | 28.440 - 2.510 |
| R-factor | 0.24057 |
| Rwork | 0.240 |
| R-free | 0.25408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1JUC |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.828 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.440 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.096 | 0.304 |
| Number of reflections | 1864 | |
| <I/σ(I)> | 4.1 | 1.4 |
| Completeness [%] | 92.9 | 90.1 |
| Redundancy | 2.84 | 3.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1mM DNA, 50mM Sodium Cacodylate buffer, 1mM CoCl2, 10mM spermine, 50% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
