3HRY
Crystal structure of PHD in a trigonal space group and partially disordered
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 71.951, 71.951, 68.007 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 11.810 - 2.250 |
| R-factor | 0.1948 |
| Rwork | 0.192 |
| R-free | 0.25630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dd7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.597 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.810 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.074 | 0.248 |
| Number of reflections | 9720 | |
| <I/σ(I)> | 14.9 | 6.48 |
| Completeness [%] | 98.3 | 93.1 |
| Redundancy | 14.3 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10% PEG 8000, 8% Ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
