3HRW
Crystal structure of hemoglobin from mouse (Mus musculus)at 2.8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-08 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.081, 65.940, 150.931 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.800 |
| R-factor | 0.24547 |
| Rwork | 0.243 |
| R-free | 0.30561 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hab |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.572 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.940 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 12720 | |
| <I/σ(I)> | 2.8 | 1 |
| Completeness [%] | 93.0 | 90 |
| Redundancy | 2.61 | 1.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
