3HRV
Crystal structure of TcpA, a Type IV pilin from Vibrio cholerae El Tor biotype
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2003-03-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 62.767, 94.752, 128.422 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| Rwork | 0.219 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oqv |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.068 | 0.413 |
| Number of reflections | 61281 | |
| <I/σ(I)> | 23.746 | 4.95 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 6.8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Na-HEPES, 1.5 M LiSO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
