3HRA

Crystal Structure of EF0377 an Ankyrin Repeat Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-02
Wavelength(s)	0.9755
Spacegroup name	P 21 21 21
Unit cell lengths	26.961, 66.902, 94.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.551 - 1.693
R-factor	0.161
Rwork	0.158
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1NFI Chain E
RMSD bond length	0.008
RMSD bond angle	1.026
Data reduction software	MOSFLM
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.551	1.799
High resolution limit [Å]	1.690	1.693
Number of reflections	16577
<I/σ(I)>	3.9
Completeness [%]	83.3
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	PEG 4K, 100mM Tris, 200mM Sodium Acetate Trihydrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K