3HQX
Crystal structure of protein of unknown function (DUF1255,PF06865) from Acinetobacter sp. ADP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-14 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.050, 25.952, 55.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.500 - 1.660 |
| R-factor | 0.16471 |
| Rwork | 0.163 |
| R-free | 0.20150 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.596 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.500 | 1.720 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.040 | 0.115 |
| Number of reflections | 11471 | |
| <I/σ(I)> | 51 | 13 |
| Completeness [%] | 98.4 | 95.3 |
| Redundancy | 4.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 25% Peg 3350 .2 M Lithium sulfate 0.1 M Hepes pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
