3HQL
Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases:SPOPMATHx-PucSBC1_pep2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Detector technology | CCD |
| Collection date | 2008-03-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.795, 43.690, 86.819 |
| Unit cell angles | 90.00, 107.03, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.660 |
| R-factor | 0.24721 |
| Rwork | 0.247 |
| R-free | 0.25806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hqh |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.363 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Number of reflections | 38876 | |
| <I/σ(I)> | 28.9 | 3.1 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
