3HQI

Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases: SPOPMATHx/BTB/3-box-PucSBC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Collection date	2008-07-31
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	57.230, 106.870, 130.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.620
Rwork	0.226
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hqh
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.710
High resolution limit [Å]	2.620	2.620
Number of reflections	24800
<I/σ(I)>	20	3.7
Redundancy	4.6	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5		pH 5.5, VAPOR DIFFUSION, HANGING DROP