3HQG
Crystal structure of restriction endonuclease EcoRII catalytic C-terminal domain in complex with cognate DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-13 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8080 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.054, 57.971, 61.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.840 - 2.600 |
R-factor | 0.242 |
Rwork | 0.236 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1na6 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.012 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.19) |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.836 | 47.840 | 2.740 |
High resolution limit [Å] | 2.110 | 8.220 | 2.600 |
Rmerge | 0.047 | 0.028 | 0.330 |
Total number of observations | 3114 | 14992 | |
Number of reflections | 8835 | ||
<I/σ(I)> | 12.41 | 18.4 | 2.3 |
Completeness [%] | 99.8 | 98 | 99.8 |
Redundancy | 11.7 | 9.7 | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 26% PEG1500, 25% glycerol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |